Article
Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol.
PloS one - 1 Jan 2016
Mogollon Catherin Marin, van Pul Fiona J A, Imai Takashi, Ramesar Jai, Chevalley-Maurel Séverine, de Roo Guido M, Veld Sabrina A J, Kroeze Hans, Franke-Fayard Blandine M D, Janse Chris J, Khan Shahid M
Abstract excerpt
The CRISPR/Cas9 system is a powerful genome editing technique employed in a wide variety of organisms including recently the human malaria parasite, P. falciparum. Here we report on further improvements to the CRISPR/Cas9 transfection constructs and selection protocol to more rapidly modify the P. falciparum genome and to introduce transgenes into the parasite genome without the inclusion of drug-selectable...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.