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Recombinase amplified CRISPR enhanced chain reaction (RACECAR) for viral genome detection

1 Jan 2022

Abstract excerpt

We have developed a 'recombinase amplified CRISPR enhanced chain reaction' (RACECAR) assay that can detect as little as 40 copies of hepatitis B virus (HBV) genome using a benchtop spectrofluorometer. The limit of detection was determined to be 3 copies of HBV genome. The specificity of RACECAR was confirmed against hepatitis A virus (HAV). This assay can detect the genomic targets directly in serum samples...

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PMID: 36102688
DOI: 10.1039/d2nr03590a

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