CRISPR-Cas12a-based ultrasensitive assay for visual detection of SARS-CoV-2 RNA

Abstract excerpt

The development of ultrasensitive and visual methods is of great significance for molecular diagnosis at the point-of-care. In this study, we have integrated recombinase polymerase amplification (RPA) with the CRISPR-Cas12a system to design an ultrasensitive strategy for visual nucleic acid testing. RPA is utilized to amplify the target nucleic acid, producing amplicons that activate the single-stranded DNase...

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• CRISPR and Genetic Engineering
• SARS-CoV-2 and COVID-19 Research
• SARS-CoV-2 detection and testing