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Article

Aberrant splicing events caused by insertion of genes of interest into expression vectors.

International journal of biological sciences - 1 Jan 2022

Cheng Yun, Kang Xiao-Zhuo, Chan Pearl, Ye Zi-Wei, Chan Chi-Ping, Jin Dong-Yan

Abstract excerpt

Background: Expression of genes of interest from plasmids or lentiviral vectors is one of the most common tools in molecular and gene therapy. Aberrant splicing between the inserted gene of interest and downstream vector sequence has not been systematically analyzed. Methods: Formation of aberrant fusion transcripts and proteins was detected by RT-PCR, sequencing, Western blotting and mass spectrometry....

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Identifiers

PMID: 35982889
DOI: 10.7150/ijbs.72408
PMCID: PMC9379414

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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