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Article

Genetics for Pseudoalteromonas provides tools to manipulate marine bacterial virus PM2.

Journal of bacteriology - 1 Feb 2008

Kivelä Hanna M, Madonna Stefania, Krupovìc Mart, Tutino M Luisa, Bamford Jaana K H

Abstract excerpt

The genetic manipulation of marine double-stranded DNA (dsDNA) bacteriophage PM2 (Corticoviridae) has been limited so far. The isolation of an autonomously replicating DNA element of Pseudoalteromonas haloplanktis TAC125 and construction of a shuttle vector replicating in both Escherichia coli and Pseudoalteromonas enabled us to design a set of conjugative shuttle plasmids encoding tRNA suppressors for amber...

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Identifiers

PMID: 18083813
DOI: 10.1128/JB.01639-07
PMCID: PMC2238219

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