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Article

Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans.

STAR protocols - 16 Sept 2022

Turan Merve G, Kantarci Hanife, Temtek Sadiye D, Cakici Onur, Cevik Sebiha, Kaplan Oktay I

Abstract excerpt

Here, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post-microscopy analysis with kymographs. This protocol breaks down all processes into three categories: (1) pre-imaging preparations, (2) preparations for the time of image acquisition,...

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Identifiers

PMID: 35776634
DOI: 10.1016/j.xpro.2022.101498
PMCID: PMC9250072

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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