Protocol to measure bacterial intake and gut clearance of Caenorhabditis elegans

Abstract excerpt

The nematode Caenorhabditis elegans is a common model organism for studies in immunity and host-pathogen interactions. In this protocol, we describe techniques for measuring pharyngeal pumping and defecation rates of adult C. elegans exposed to either pathogenic or non-pathogenic bacteria. Quantifying changes in pumping or defecation rates during pathogenic infection can account for behavioral changes that may...

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