Article
Measuring rates of intraflagellar transport along Caenorhabditis elegans sensory cilia using fluorescence microscopy.
Methods in enzymology - 1 Jan 2013
Brust-Mascher Ingrid, Ou Guangshuo, Scholey Jonathan M
Abstract excerpt
Intraflagellar transport (IFT), the kinesin-2 and IFT-dynein-dependent bidirectional movement of multisubunit protein complexes called IFT-particles and associated cargo molecules along ciliary axonemes, is thought to be essential for the assembly and maintenance of virtually all eukaryotic cilia and flagella. Transport assays that allow measurements of the rates of movement of specific, fluorescently tagged,...
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