Article
The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes.
Cell stem cell - 5 May 2022
Ma Haiting, de Zwaan Esmée, Guo Yang Eric, Cejas Paloma, Thiru Prathapan, van de Bunt Martijn, Jeppesen Jacob F, Syamala Sudeepa, Dall'Agnese Alessandra, Abraham Brian J, Fu Dongdong, Garrett-Engele Carrie, Lee Tong Ihn, Long Henry W, Griffith Linda G, Young Richard A, Jaenisch Rudolf
Abstract excerpt
To understand the mechanisms regulating the in vitro maturation of hPSC-derived hepatocytes, we developed a 3D differentiation system and compared gene regulatory elements in human primary hepatocytes with those in hPSC-hepatocytes that were differentiated in 2D or 3D conditions by RNA-seq, ATAC-seq, and H3K27Ac ChIP-seq. Regulome comparisons showed a reduced enrichment of thyroid receptor THRB motifs in...
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