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Article

An in planta biolistic method for stable wheat transformation.

Scientific reports - 13 Sept 2017

Hamada Haruyasu, Linghu Qianyan, Nagira Yozo, Miki Ryuji, Taoka Naoaki, Imai Ryozo

Abstract excerpt

The currently favoured method for wheat (Triticum aestivum L.) transformation is inapplicable to many elite cultivars because it requires callus culture and regeneration. Here, we developed a simple, reproducible, in planta wheat transformation method using biolistic DNA delivery without callus culture or regeneration. Shoot apical meristems (SAMs) grown from dry imbibed seeds were exposed under a microscope and...

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Identifiers

PMID: 28904403
DOI: 10.1038/s41598-017-11936-0
PMCID: PMC5597576

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