Article
A novel two T-DNA binary vector allows efficient generation of marker-free transgenic plants in three elite cultivars of rice (Oryza sativa L.).
Transgenic research - 1 Jun 2004
Breitler Jean-Christophe, Meynard Donaldo, Van Boxtel Jos, Royer Monique, Bonnot François, Cambillau Laurence, Guiderdoni Emmanuel
Abstract excerpt
A pilot binary vector was constructed to assess the potential of the 2 T-DNA system for generating selectable marker-free progeny plants in three elite rice cultivars (ZhongZuo321, Ariete and Khao Dawk Mali 105) known to exhibit contrasting amenabilities to transformation. The first T-DNA of the vector, delimited by Agrobacterium tumefaciens borders, contains the hygromycin phosphotransferase (hpt) selectable...
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