Article
Quantification of mutated alleles of 23S rRNA in macrolide-resistant Campylobacter by TaqMan real-time polymerase chain reaction.
Foodborne pathogens and disease - 1 Jan 2010
Hao Haihong, Dai Menghong, Wang Yulian, Chen Dongmei, Yuan Zonghui
Abstract excerpt
A quantitative detection of mutations mediating high-level macrolide resistance in Campylobacter is necessary for molecular epidemiological investigation and research. In this study, a new TaqMan probe-based real-time polymerase chain reaction method, with high specificity and sensitivity, was firstly established to identify and quantify mutated alleles associated with macrolide resistance (A2074C and A2075G) in...
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