Article

Development and Evaluation of Real Time PCR for Detection and Identification of C. jejuni and C. coli from Human Food

2022-06-22

Abstract excerpt

The current study aimed to develop, optimize, and evaluate the real-time polymerase chain reaction (qPCR) specificity and sensitivity on pure isolated strains of Campylobacter and on enriched broth for direct detection of these cells. To achieve this, HipO and CadF gene were purchased to target C. jejuni and C. coli , respectively. Using chessboard titration method, three critical reagents concentration of qPCR we...

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Literature Corpus work: 9cba8ef5-828f-5a1a-b0e9-25c53eb67e1d
DOI: 10.21203/rs.3.rs-1758442/v1

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