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Article

Deciphering the role of the conjugate's phycoerythrin label in complement-mediated interference occurring in HLA single antigen Luminex bead assays.

HLA - 1 Jun 2022

Devriese Magali, Hays Constantin, Jouffrey Julie, Usureau Cédric, Carmagnat Maryvonnick, Caillat-Zucman Sophie, Taupin Jean Luc

Abstract excerpt

Complement-mediated interference is a well described phenomenon in single antigen bead (SAB) Luminex assay that leads to falsely low or negative results for anti-HLA antibody (Ab). In a context of high amount of Ab, the enrichment of the Ab around the bead can lead to complement cascade activation and deposition, thereafter impairing Ab detection. EDTA is now routinely used to circumvent this interference. In...

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PMID: 35411722
DOI: 10.1111/tan.14628

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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