Article
A modeling approach for mean fluorescence intensity value harmonization and cutoff prediction for luminex single antigen bead assays of two different vendors.
HLA - 1 Nov 2023
Karahan Gonca E, Haasnoot Geert W, Voogt-Bakker Kim, Claas Frans H J, Roelen Dave, Heidt Sebastiaan
Abstract excerpt
Luminex single antigen bead (SAB) kits from One Lambda (OL) and Lifecodes (LC) are widely used for HLA antibody detection but have substantial differences in design and assay protocol resulting in different mean fluorescence intensity (MFI) values. Here, we present a non-linear modeling approach to accurately convert MFI values between two vendors and to establish user-independent MFI cutoffs when analyzing big...
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