Article
A saturation-mutagenesis analysis of the interplay between stability and activation in Ras.
eLife - 11 Mar 2022
Hidalgo Frank, Nocka Laura M, Shah Neel H, Gorday Kent, Latorraca Naomi R, Bandaru Pradeep, Templeton Sage, Lee David, Karandur Deepti, Pelton Jeffrey G, Marqusee Susan, Wemmer David, Kuriyan John
Abstract excerpt
Cancer mutations in Ras occur predominantly at three hotspots: Gly 12, Gly 13, and Gln 61. Previously, we reported that deep mutagenesis of H-Ras using a bacterial assay identified many other activating mutations (Bandaru et al., 2017). We now show that the results of saturation mutagenesis of H-Ras in mammalian Ba/F3 cells correlate well with the results of bacterial experiments in which H-Ras or K-Ras are...
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