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DNA isolation methods for Nanopore sequencing of the Streptococcus mitis genome

16 Feb 2022

Abstract excerpt

Streptococcus mitis is a Gram-positive bacterium, member of the oral commensal microbiota, which can occasionally be the etiologic agent of diseases such as infective endocarditis, bacteraemia and septicaemia. The highly recombinogenic and repetitive nature of the S. mitis genome impairs the assembly of a complete genome relying only on short sequencing reads. Oxford Nanopore sequencing can overcome this...

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PMID: 35171093
DOI: 10.1099/mgen.0.000764

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