Article
In situ T-cell transfection by anti-CD3-conjugated lipid nanoparticles leads to T-cell activation, migration, and phenotypic shift.
Biomaterials - 1 Feb 2022
Kheirolomoom Azadeh, Kare Aris J, Ingham Elizabeth S, Paulmurugan Ramasamy, Robinson Elise R, Baikoghli Mo, Inayathullah Mohammed, Seo Jai W, Wang James, Fite Brett Z, Wu Bo, Tumbale Spencer K, Raie Marina N, Cheng R Holland, Nichols Lisa, Borowsky Alexander D, Ferrara Katherine W
Abstract excerpt
Ex vivo programming of T cells can be efficacious but is complex and expensive; therefore, the development of methods to transfect T cells in situ is important. We developed and optimized anti-CD3-targeted lipid nanoparticles (aCD3-LNPs) to deliver tightly packed, reporter gene mRNA specifically to T cells. In vitro, targeted LNPs efficiently delivered mCherry mRNA to Jurkat T cells, and T-cell activation and...
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