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Article

Highly efficient CRISPR-Cas9-mediated editing identifies novel mechanosensitive microRNA-140 targets in primary human articular chondrocytes

21 Jan 2022

Abstract excerpt

Objective MicroRNA 140 ( miR-140) is a chondrocyte-specific endogenous gene regulator implicated in osteoarthritis (OA). As mechanical injury is a primary aetiological factor in OA, we investigated miR-140 -dependent mechanosensitive gene regulation using a novel CRISPR-Cas9 methodology in primary human chondrocytes. Method Primary (passage 1/2) human OA chondrocytes were isolated from arthroplasty samples (six...

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PMID: 35074547
DOI: 10.1016/j.joca.2022.01.005

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