Article
Impact of Cryopreservation on Viability, Phenotype, and Functionality of Porcine PBMC.
Frontiers in immunology - 1 Jan 2021
Li Yanli, Mateu Enric, Díaz Ivan
Abstract excerpt
The use of frozen peripheral blood mononuclear cells (PBMC) is common in immunological studies. The impact of freezing PBMC has been assessed using human and mice cells, but little information is available regarding domestic animals. In the present study, the phenotype and functionality of frozen porcine PBMC were examined. In a preliminary experiment, three freezing media: fetal bovine serum plus 10% dimethyl...
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