Article

Addition of DMSO to T cell cultures skews differentiation towards a memory phenotype

2026-03-17

Abstract excerpt

Dimethyl sulfoxide (DMSO) is a polar aprotic organic solvent that is widely used in biological applications. It is routinely applied as a cryoprotectant for long-term cell freezing as well as to dissolve peptides or drugs for immune cell functional assays. We report on a remarkable impact of low concentrations of DMSO on in vitro expansion of the total CD3 + pool isolated from human PBMCs as well as on the purif...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7ea29eff-5489-5b00-a767-78802a7ac19e
DOI: 10.64898/2026.03.13.711393

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Addition of DMSO to T cell cultures skews differentiation towards a memory phenotypeDOI 10.64898/2026.03.13.711393Read

Effects of Different Media on Human T Regulatory Cells Phenotype.Semantic proximity Read

Phenotypic and functional comparisons between cryopreserved and freshly isolated peripheral blood mononuclear cells with or without red blood cell lysate (ACK) treatment with special focus on regulatory T cells.Semantic proximity Read

A Practical Cryopreservation and Staining Protocol for Immunophenotyping in Population StudiesSemantic proximity Read

Strong Expansion of Human Regulatory T Cells for Adoptive Cell Therapy Results in Epigenetic Changes Which May Impact Their Survival and FunctionSemantic proximity Read

Human and Murine T-Helper Cell Recovery from Organs and Tissues.Semantic proximity Read

Effects of long-term cryopreservation of PBMC on recovery of B cell subpopulations.Semantic proximity Read

Standard Peripheral Blood Mononuclear Cell Cryopreservation Selectively Decreases Detection of Nine Clinically Relevant T Cell MarkersSemantic proximity Read

Identification and Purification of Human Memory T Helper Cells from Peripheral Blood.Semantic proximity Read

Biological and Clinical Applications of Dimethyl Sulfoxide: Evidence and Safety ConsiderationsSemantic proximity Read

CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.Semantic proximity Read

Oligodeoxynucleotides stabilize Helios-expressing Foxp3+ human T regulatory cells during in vitro expansionSemantic proximity Read

A global look into human T cell subsets before and after cryopreservation using multiparametric flow cytometry and two-dimensional visualization analysisSemantic proximity Read