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Article

Establishment of quantitative RNAi-based forward genetics in Entamoeba histolytica and identification of genes required for growth.

PLoS pathogens - 1 Nov 2021

Bettadapur Akhila, Hunter Samuel S, Suleiman Rene L, Ruyechan Maura C, Huang Wesley, Barbieri Charles G, Miller Hannah W, Tam Tammie S Y, Settles Matthew L, Ralston Katherine S

Abstract excerpt

While Entamoeba histolytica remains a globally important pathogen, it is dramatically understudied. The tractability of E. histolytica has historically been limited, which is largely due to challenging features of its genome. To enable forward genetics, we constructed and validated the first genome-wide E. histolytica RNAi knockdown mutant library. This library allows for Illumina deep sequencing analysis for...

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Identifiers

PMID: 34843592
DOI: 10.1371/journal.ppat.1010088
PMCID: PMC8716031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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