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Article

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping.

BMC genomics - 24 Nov 2021

Li Baowei, Liu Yanran, Hao Xiaodan, Dong Jinhua, Chen Limei, Li Haimei, Wu Wei, Liu Ying, Wang Jianxun, Wang Yin, Li Peifeng

Abstract excerpt

BACKGROUND: The detection and identification of single nucleotide polymorphism (SNP) is essential for determining patient disease susceptibility and the delivery of medicines targeted to the individual. At present, SNP genotyping technology includes Sanger sequencing, TaqMan-probe quantitative polymerase chain reaction (qPCR), amplification-refractory mutation system (ARMS)-PCR, and Kompetitive Allele-Specific...

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PMID: 34819030
DOI: 10.1186/s12864-021-08148-2
PMCID: PMC8611915

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