Article
Expression level is a major modifier of the fitness landscape of a protein coding gene.
Nature ecology & evolution - 1 Jan 2022
Wu Zhuoxing, Cai Xiujuan, Zhang Xin, Liu Yao, Tian Guo-Bao, Yang Jian-Rong, Chen Xiaoshu
Abstract excerpt
The phenotypic consequence of a genetic mutation depends on many factors including the expression level of a gene. However, a comprehensive quantification of this expression effect is still lacking, as is a further general mechanistic understanding of the effect. Here, we measured the fitness effect of almost all (>97.5%) single-nucleotide mutations in GFP, an exogenous gene with no physiological function, and...
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