Article

Discrepancies in the detection of SARS-CoV-2 by qRT-PCR are dependent on the target gene used for its amplification: Implications in the diagnosis of clinical infection

2021-08-31

Abstract excerpt

Discrepancies exist in Cycle threshold (Ct) values during detection of SARS-CoV-2 by qRT-PCR. We demonstrate that Ct values depend on the position of the target gene in the viral genome. Simultaneous detection of five genes in positive samples revealed lower Ct values as we move further to the 3’ end (orf1AB/RdRp>E>M>orf7a>N). These findings were confirmed in a retrospective analysis with 363 positive clinical sam...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 39d583ac-d13c-50f2-b44a-1c9ee3ec32ff
DOI: 10.1101/2021.08.30.21262536

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Discrepancies in the detection of SARS-CoV-2 by qRT-PCR are dependent on the target gene used for its amplification: Implications in the diagnosis of clinical infectionDOI 10.1101/2021.08.30.21262536Read

Comparison and Sensitivity Evaluation of Three Different Commercial Real-Time Quantitative PCR Kits for SARS-CoV-2 DetectionSemantic proximity Read

Inclusion of cycle threshold (CT) values when reporting SARS-CoV-2 RT-PCR results improves clinical Interpretation in suspected and confirmed COVID-19Semantic proximity Read

Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold valueSemantic proximity Read

True or false: what are the factors that influence COVID-19 diagnosis by RT-qPCR?Semantic proximity Read

Implication of SARS-CoV-2 evolution in the sensitivity of RT-qPCR diagnostic assaysSemantic proximity Read

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection? Semantic proximity Read

Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunitiesSemantic proximity Read

Variability of cycle threshold values in an external quality assessment scheme for detection of the SARS-CoV-2 virus genome by RT-PCRSemantic proximity Read

Detection of SARS-CoV-2 in Clinical Samples: Target-specific Analysis of Qualitative Reverse Transcription–Polymerase Chain Reaction(RT-PCR) Diagnostic KitsSemantic proximity Read

Evaluation of WHO listed COVID-19 qPCR primers and probe in silico with 375 SERS-CoV-2 full genome sequencesSemantic proximity Read

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?Semantic proximity Read

A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2Semantic proximity Read