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Article

Solid-Phase Primer Elongation Using Biotinylated dNTPs for the Detection of a Single Nucleotide Polymorphism from a Fingerprick Blood Sample.

Analytical chemistry - 9 Nov 2021

Jauset-Rubio Miriam, Ortiz Mayreli, O'Sullivan Ciara K

Abstract excerpt

Isothermal recombinase polymerase amplification-based solid-phase primer extension is used for the optical detection of a hypertrophic cardiomyopathy associated single nucleotide polymorphism (SNP) in a fingerprick blood sample. The assay exploits four thiolated primers which have the same sequences with the exception of the 3'-terminal base. Target DNA containing the SNP site hybridizes to all four of the...

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PMID: 34704755
DOI: 10.1021/acs.analchem.1c03419
PMCID: PMC8581964

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