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Article

Enhancing protein perdeuteration by experimental evolution of Escherichia coli K-12 for rapid growth in deuterium-based media.

Protein science : a publication of the Protein Society - 1 Dec 2021

Kelpšas Vinardas, von Wachenfeldt Claes

Abstract excerpt

Deuterium is a natural low abundance stable hydrogen isotope that in high concentrations negatively affects growth of cells. Here, we have studied growth of Escherichia coli MG1655, a wild-type laboratory strain of E. coli K-12, in deuterated glycerol minimal medium. The growth rate and final biomass in deuterated medium is substantially reduced compared to cells grown in ordinary medium. By using a...

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PMID: 34655136
DOI: 10.1002/pro.4206
PMCID: PMC8605374

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