Article
Adaptation of an in-house PCR for the detection of Helicobacter pylori and the mutations associated with macrolide resistance into ready-to-use PCR microwell strips.
Helicobacter - 1 Dec 2021
Bénéjat Lucie, Ducournau Astrid, Domingues-Martins Chloé, Lecoeur Mélanie, Blosse Alice, Mégraud Francis, Bessède Emilie, Lehours Philippe
Abstract excerpt
BACKGROUND AND OBJECTIVES: The present study describes the successful adaptation of an in-house Polymerase Chain Reaction (PCR) for Helicobacter pylori detection coupled with the main mutations associated with resistance to clarithromycin in ready-to-use PCR microwell strips. MATERIALS AND METHODS: These microwell strips can be used on LightCycler® 480, and are delivered with nine microliters of the reaction...
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