Article
Isolation, DNA sequence analysis, and mutagenesis of a proline dehydrogenase gene (putA) from Bradyrhizobium japonicum.
Applied and environmental microbiology - 1 Jan 1996
Straub P F, Reynolds P H, Althomsons S, Mett V, Zhu Y, Shearer G, Kohl D H
Abstract excerpt
We report here the cloning and sequencing of the gene for proline dehydrogenase (putA) of Bradyrhizobium japonicum. An open reading frame coding for 1,016 amino acids was identified. The B. japonicum gene codes for a bifunctional protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C...
Topics
- 1-Pyrroline-5-Carboxylate Dehydrogenase
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Genes, Bacterial
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Oxidoreductases Acting on CH-NH Group Donors
- Proline Oxidase
- Restriction Mapping