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Article

Kinetic and isotopic characterization of L-proline dehydrogenase from Mycobacterium tuberculosis.

Biochemistry - 23 Jul 2013

Serrano Hector, Blanchard John S

Abstract excerpt

The monofunctional proline dehydrogenase (ProDH) from Mycobacterium tuberculosis performs the flavin-dependent oxidation of l-proline to Δ(1)-pyrroline-5-carboxylate in the proline catabolic pathway. The ProDH gene, prub, was cloned into the pYUB1062 vector, and the C-terminal His-tagged 37 kDa protein was expressed and purified by nickel affinity chromatography. A steady-state kinetic analysis revealed a...

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Identifiers

PMID: 23834473
DOI: 10.1021/bi400338f
PMCID: PMC3839249

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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