Article
ROTADIAL: The first nanobody-based immunoassay to detect Group A Rotavirus.
Journal of virological methods - 1 Dec 2021
Vega C G, Garaicoechea L L, Degiuseppe J I, Bok M, Rivolta A A, Piantanida A P, Asenzo G, Adúriz Guerrero M, Wigdorovitz A, Stupka J A, Parreño V
Abstract excerpt
ROTADIAL is a rapid nanobody (Nb)-based ELISA assay able to identify Rotavirus group A (RVA) in feces from pediatric patients. The assay is based on a sandwich of two patented llama-derived Nbs directed to the inner capsid viral protein VP6 from RVA. Nbs are directed to conformational epitopes of VP6 and recognized all human RVA strains tested, representing ideal reagents for their use in immunodiagnostic tests...
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