Article
A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection.
BMC genomics - 24 Apr 2017
Dung Tran Thi Ngoc, Duy Pham Thanh, Sessions October M, Sangumathi Uma K, Phat Voong Vinh, Tam Pham Thi Thanh, To Nguyen Thi Nguyen, Phuc Tran My, Hong Chau Tran Thi, Chau Nguyen Ngoc Minh, Minh Ngoc Nguyen, Thwaites Guy E, Rabaa Maia A, Baker Stephen
Abstract excerpt
BACKGROUND: Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material. METHODS: To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents. RESULTS: Our...
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