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Article

HygY Is a Twitch Radical SAM Epimerase with Latent Dehydrogenase Activity Revealed upon Mutation of a Single Cysteine Residue.

Journal of the American Chemical Society - 22 Sept 2021

Besandre Ronald A, Chen Zhang, Davis Ian, Zhang Jiawei, Ruszczycky Mark Walter, Liu Aimin, Liu Hung-Wen

Abstract excerpt

HygY is a SPASM/twitch radical SAM enzyme hypothesized to catalyze the C2'-epimerization of galacamine during the biosynthesis of hygromycin B. This activity is confirmed via biochemical and structural analysis of the derivatized reaction products using chemically synthesized deuterated substrate, high-resolution mass spectrometry and 1H NMR. Electron paramagnetic resonance spectroscopy of the reduced enzyme is...

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Identifiers

PMID: 34491039
DOI: 10.1021/jacs.1c05727
PMCID: PMC8611816

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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