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A Genome-Wide CRISPR/Cas9 Screen Reveals the Requirement of Host Sphingomyelin Synthase 1 for Infection with Pseudorabies Virus Mutant gD-Pass.

Viruses - 9 Aug 2021

Hölper Julia E, Grey Finn, Baillie John Kenneth, Regan Tim, Parkinson Nicholas J, Höper Dirk, Thamamongood Thiprampai, Schwemmle Martin, Pannhorst Katrin, Wendt Lisa, Mettenleiter Thomas C, Klupp Barbara G

Abstract excerpt

Herpesviruses are large DNA viruses, which encode up to 300 different proteins including enzymes enabling efficient replication. Nevertheless, they depend on a multitude of host cell proteins for successful propagation. To uncover cellular host factors important for replication of pseudorabies virus (PrV), an alphaherpesvirus of swine, we performed an unbiased genome-wide CRISPR/Cas9 forward screen. To this end,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34452438
DOI: 10.3390/v13081574
PMCID: PMC8402627

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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