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Article

Development of a specific live-cell assay for native autophagic flux.

The Journal of biological chemistry - 1 Sept 2021

Safren Nathaniel, Tank Elizabeth M, Malik Ahmed M, Chua Jason P, Santoro Nicholas, Barmada Sami J

Abstract excerpt

Autophagy is an evolutionarily conserved pathway mediating the breakdown of cellular proteins and organelles. Emphasizing its pivotal nature, autophagy dysfunction contributes to many diseases; nevertheless, development of effective autophagy modulating drugs is hampered by fundamental deficiencies in available methods for measuring autophagic activity or flux. To overcome these limitations, we introduced the...

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Identifiers

PMID: 34303705
DOI: 10.1016/j.jbc.2021.101003
PMCID: PMC8368035

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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