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Oxygen concentration affects de novo DNA methylation and transcription in in vitro cultured oocytes

28 Jun 2021

Abstract excerpt

Abstract Background Reproductive biology methods rely on in vitro follicle cultures from mature follicles obtained by hormonal stimulation for generating metaphase II oocytes to be fertilised and developed into a healthy embryo. Such techniques are used routinely in both rodent and human species. DNA methylation is a dynamic process that plays a role in epigenetic regulation of gametogenesis and development. In...

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PMID: 34183052
DOI: 10.1186/s13148-021-01116-3

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