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Article

Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Correia Bibiana, Sousa Maria Inês, Branco Ana F, Ramalho-Santos João

Abstract excerpt

Mouse embryonic stem cells (mESCs) can be grown in culture, recapitulating the different states of pluripotency of their in vivo counterparts, with notably different metabolic profiles. mESCs in a naïve pluripotent state present an ambivalent metabolism, using both glycolysis and oxidative phosphorylation as energy sources. Here, we describe a method to evaluate the oxidative function of naïve mESCs using the...

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PMID: 34095997
DOI: 10.1007/978-1-0716-1433-4_4

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