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Article

Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics.

Stem cells (Dayton, Ohio) - 1 Oct 2013

Grosselin Jeanne, Sii-Felice Karine, Payen Emmanuel, Chretien Stany, Tronik-Le Roux Diana, Leboulch Philippe

Abstract excerpt

Our understanding of system dynamics of mixed cell populations in whole organisms has benefited from the advent of individual cell marking by nonarrayed DNA barcodes subsequently analyzed by high-throughput DNA sequencing. However, key limitations include statistical biases compromising quantification and the lack of applicability to deconvolute individual cell fate in vivo after pooling single cells...

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PMID: 23554255
DOI: 10.1002/stem.1383

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