Article
Human MC4R variants affect endocytosis, trafficking and dimerization revealing multiple cellular mechanisms involved in weight regulation.
Cell reports - 23 Mar 2021
Brouwers Bas, de Oliveira Edson Mendes, Marti-Solano Maria, Monteiro Fabiola B F, Laurin Suli-Anne, Keogh Julia M, Henning Elana, Bounds Rebecca, Daly Carole A, Houston Shane, Ayinampudi Vikram, Wasiluk Natalia, Clarke David, Plouffe Bianca, Bouvier Michel, Babu M Madan, Farooqi I Sadaf, Mokrosiński Jacek
Abstract excerpt
The Melanocortin-4 Receptor (MC4R) plays a pivotal role in energy homeostasis. We used human MC4R mutations associated with an increased or decreased risk of obesity to dissect mechanisms that regulate MC4R function. Most obesity-associated mutations impair trafficking to the plasma membrane (PM), whereas obesity-protecting mutations either accelerate recycling to the PM or decrease internalization, resulting in...
Topics
- Animals
- Body Weight
- COS Cells
- Cell Membrane
- Chlorocebus aethiops
- Cyclic AMP
- Endocytosis
- Extracellular Signal-Regulated MAP Kinases
- GTP-Binding Protein alpha Subunits, Gs