Article
Characterization of microbial antifreeze protein with intermediate activity suggests that a bound-water network is essential for hyperactivity.
Scientific reports - 16 Mar 2021
Khan N M-Mofiz Uddin, Arai Tatsuya, Tsuda Sakae, Kondo Hidemasa
Abstract excerpt
Antifreeze proteins (AFPs) inhibit ice growth by adsorbing onto specific ice planes. Microbial AFPs show diverse antifreeze activity and ice plane specificity, while sharing a common molecular scaffold. To probe the molecular mechanisms responsible for AFP activity, we here characterized the antifreeze activity and crystal structure of TisAFP7 from the snow mold fungus Typhula ishikariensis. TisAFP7 exhibited...
Topics
- Amino Acid Sequence
- Antifreeze Proteins
- Basidiomycota
- Binding Sites
- Fungal Proteins
- Ice
- Liquid Crystals
- Molecular Docking Simulation
- Molecular Dynamics Simulation
- Mutation
- Protein Binding
- Protein Conformation
- Protein Interaction Domains and Motifs
- Structure-Activity Relationship