Article
Construction and characterization of a reovirus double temperature-sensitive mutant.
Proceedings of the National Academy of Sciences of the United States of America - 24 Jun 1997
Roner M R, Nepliouev I, Sherry B, Joklik W K
Abstract excerpt
The infectious reovirus RNA system was used to construct a mutant with two temperature-sensitive (ts) lesions in genome segments M2 and S2, respectively. The double mutant is about 300 times more ts than either of its parents, which are about 1,500 and 170 times more ts than their wild-type parent reovirus ST3 strain Dearing. At 39 degrees C the double mutant is essentially unable to multiply. In spite of its...
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