Article
Effects of the phenotypic polarization state of human leukocytes on the optical absorbance spectrum.
Journal of biophotonics - 1 Jun 2021
de Brito Sousa Kaline, de Fátima Teixeira da Silva Daniela, Rodrigues Maria Fernanda Setúbal Destro, Garcia Mónica Pereira, de Oliveira Rodini Carolina, Mesquita-Ferrari Raquel Agnelli, Hamblin Michael R, Bussadori Sandra Kalil, Nunes Fabio Daumas, Fernandes Kristianne Porta Santos
Abstract excerpt
This study evaluated the optical absorbance spectrum of human monocytes, neutrophils and lymphocytes polarized, or not, to the inflammatory or immunoregulatory phenotypes. Peripheral human blood leukocytes were isolated and polarized (10 ng/mL) with LPS or IL-4 + LPS for 2 hours. After polarization, cells were washed and incubated for an additional 24 hours (monocytes and lymphocytes) or 12 hours (neutrophils)....
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