Article
Detection of the SARS-CoV-2 D614G mutation using engineered Cas12a guide RNA.
Biotechnology journal - 1 Jun 2021
Meng Qingzhou, Wang Xinjie, Wang Yanqun, Dang Lu, Liu Xinyi, Ma Xiaodong, Chi Tian, Wang Xian, Zhao Qin, Yang Guang, Liu Ming, Huang Xingxu, Ma Peixiang
Abstract excerpt
Detection of pathogens with single-nucleotide variations is indispensable for the disease tracing, but remains technically challenging. The D614G mutation in the SARS-CoV-2 spike protein is known to markedly enhance viral infectivity but is difficult to detect. Here, we report an effective approach called "synthetic mismatch integrated crRNA guided Cas12a detection" (symRNA-Cas12a) to detect the D614 and G614...
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