Article
Chimeric crRNA improves CRISPR-Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2.
PloS one - 1 Jan 2021
Yang Jun, Barua Nilakshi, Rahman Md Nannur, Lo Norman, Tsang Tsz Fung, Yang Xiao, Chan Paul K S, Zhang Li, Ip Margaret
Abstract excerpt
Many CRISPR/Cas platforms have been established for the detection of SARS-CoV-2. But the detection platform of the variants of SARS-CoV-2 is scarce because its specificity is very challenging to achieve for those with only one or a few nucleotide(s) differences. Here, we report for the first time that chimeric crRNA could be critical in enhancing the specificity of CRISPR-Cas12a detecting of N501Y, which is...
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