KPC-12 with a L169M substitution in the Ω loop has reduced carbapenemase activity.

Abstract excerpt

KPC-12 is a variant of KPC-2 with a L169M substitution in the Ω loop, but its resistance spectrum was not reported. blaKPC-12 was cloned, and KPC-12 exhibited significantly decreased activities against imipenem, meropenem, aztreonam, and piperacillin-tazobactam with ≥4-fold lower MICs than KPC-2. However, unlike the L169P substitution in KPC-35, activities against ceftazidime and ceftazidime-avibactam of KPC-12...

Topics

• Amino Acid Substitution
• Bacterial Proteins
• Drug Resistance, Multiple, Bacterial
• Gene Expression Regulation, Enzymologic
• Humans
• Klebsiella pneumoniae
• Microbial Sensitivity Tests
• Models, Molecular
• Mutation
• Protein Conformation