Article
Maltose binding site 2 mutations affect product inhibition of Bacillus circulans STB01 cyclodextrin glycosyltransferase.
International journal of biological macromolecules - 1 Apr 2021
Li Caiming, You Yuxian, Zhang Yuzhu, Xie Xiaofang, Xu Qi, Gu Zhengbiao, Ban Xiaofeng, Tang Xiaoshu, Hong Yan, Cheng Li, Li Zhaofeng
Abstract excerpt
The efficiency of enzymatic cyclodextrin production using cyclodextrin glycosyltransferases (CGTases) is limited by product inhibition. In this study, maltose binding site 2 (MBS2) of the β-CGTase from Bacillus circulans STB01 was modified to decrease product inhibition. First, two point mutants were prepared at position 599 (A599V and A599N). Then, two double mutants incorporating alanine at position 633...
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