Article
The role of arginine 47 in the cyclization and coupling reactions of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 implications for product inhibition and product specificity.
European journal of biochemistry - 1 Jun 2000
van der Veen B A, Uitdehaag J C, Dijkstra B W, Dijkhuizen L
Abstract excerpt
Cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) is used for the industrial production of cyclodextrins. Its application, however, is hampered by the limited cyclodextrin product specificity and the strong inhibitory effect of cyclodextrins on CGTase activity. Recent structural studies have identified Arg47 in the Bacillus circulans strain 251 CGTase as an active-site residue interacting with...
Topics
- Amino Acid Substitution
- Arginine
- Bacillus
- Binding Sites
- Cyclodextrins
- Enzyme Stability
- Glucosyltransferases
- Mutation
- Substrate Specificity