Article
Mutations at calcium binding site III in cyclodextrin glycosyltransferase improve β-cyclodextrin specificity.
International journal of biological macromolecules - 1 May 2015
Ban Xiaofeng, Gu Zhengbiao, Li Caiming, Huang Min, Cheng Li, Hong Yan, Li Zhaofeng
Abstract excerpt
Cyclodextrin glycosyltransferases (CGTases, EC 2.4.1.19) are industrially important enzymes that produce cyclodextrins from starch by intramolecular transglycosylation. In this study, the effects of amino acid residue at position 315 in calcium binding site III (CaIII) on product specificity of CGTase were investigated by replacing Ala315 in the CGTase from Bacillus circulans STB01 with arginine, aspartic acid,...
Topics
- Binding Sites
- Calcium
- Cyclization
- Glucosyltransferases
- Models, Molecular
- Molecular Conformation
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- Protein Interaction Domains and Motifs