[Altered Splicing in Stable Cell Strains Expressing Mini-hF9 Gene with Nonsense Mutation].

doi:10.19746/j.cnki.issn.1009-2137.2021.01.037

Article

[Altered Splicing in Stable Cell Strains Expressing Mini-hF9 Gene with Nonsense Mutation].

Zhongguo shi yan xue ye xue za zhi - 1 Feb 2021

Wang Gang, Sun Wen-Wen, Zhu Lv-Kai, Ma Yan-Chun, Zhang Xia-Lin, Zhang Jian-Hua, Ren Juan, Qin Xiu-Yu, Yang Lin-Hua, Chai Bao-Feng

Abstract excerpt

OBJECTIVE: To investigate the molecular mechanism in stable cell strains expressing Mini-hF9 gene with nonsense mutation. METHODS: Mini-hF9 gene and its nonsense mutants were transfected into HeLa cells independently, and stable cell strains were obtained after G418 resistance screening and monoclonal transformation. The altered splicing and protein expression of mRNA in Mini-hF9 gene in stable cell strains were...

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[Altered Splicing in Stable Cell Strains Expressing Mini-hF9 Gene with Nonsense Mutation].

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