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[Expression of human betaIVS- II -nt 654 C --> T allele in HeLa cell].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Oct 1997

Chen W, Gu X, Sun Q

Abstract excerpt

OBJECTIVE: To establish a cell model expressing human betaIVS- II -nt 654C --> T allele (beta654 mutant). METHODS: DNA fragment of entire beta-globin gene encompassing the codon region and poly(A) signal of the allele was amplified by long PCR from the genomic DNA of a homozygote with beta65.4 mutation. The amplified fragments were cloned into the Hind III and Xba I sites of the pcDNA3.1 vector. After...

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PMID: 15625829

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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